am.jpg (7400 bytes)   Medium

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Nutrient agar

Composition per liter:

Agar 15.0 g
Peptone         5.0 g
NaCl   5.0 g
Yest extract   2.0 g
Beef extract   1.0 g

pH 7.40.2

Source: This medium is available as a premixed powder from Oxoid.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0 L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 121oC.

Reference: Atlas, R.M. 1993. Handbook of microbiological media. Parks, L.C. (ed). CRC Press Inc., Boca Raton, 1993. P. 666.