am.jpg (7400 bytes)   Medium

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Rhodococcus Medium with propane (n-butane)

Use mineral base of Medium Kievskaya (Malashenko et al., 1973).

Composition per liter:

KNO3 1.0 g
KH2PO4 1.0 g
K2HPO4 1.0 g
NaCl 1.0 g
MgSO4 0.2 g
CaCl2 0.02 g
FeCl3 0.001 g

pH 6.8-7.0

Preparation of Medium: Sterilize liquid media at 121oC for 30 min, agarized - at 105oC for 20 min. Gas mixture composition: propane (n-butane) - air 1:5.

Reference: Malashenko, Yu.A., Romanovskaya, V.A., Bogachenko, V.N., Khotian, L.V., Voloshin, N.V. 1973. Characteristics nutrition of microorganisms growing on natural gas. Microbiologiya, 42, 3: 403-408.