Medium
6
Raymond's medium
Composition per liter:
| NH4NO3 | 2.0 g |
| KH2PO4 | 2.0 g |
| Na2HPO4 | 3.0 g |
| NaCl | 1.0-5.0 g |
| MgSO4 | 0.2 g |
| Na2CO3 | 0.1 g |
| CaCl2 | 0.01 g |
| MnSO4 | 0.02 g |
| FeSO4 | 0.01 g |
pH 6.8-7.0
Preparation of Medium: n-Hexadecane is used as the sole carbon source at a concentration of 1.0 vol. %. Sterilised at 121oC for 20 min.
Reference: Raymond, R.L. 1961. Microbial oxidation of n-paraffinic hydrocarbons. Develop. Indust. Microbiol., 2: 23-32.