Rhodococcus Surfactant Medium (RS Medium)
Composition per liter:
|MgSO4 . 7H2O||0.2 g|
|CaCl2 . 2H2O||0.02 g|
|FeCl3 . 7H2O||0.01 g|
|Trace element solution||1.0 ml|
Preparation of Medium: Yeast extract (0.1 % w/v) is added to the sterilized basal medium. Mixture of n-alkanes (C10-C16 or C12-C17) is used as the sole carbon source at a concentration of 3.0 vol. %. Autoclave liquid media at 121oC for 30 min, agarized - at 105oC for 20 min.
Reference: Ivshina, I.B., Kuyukina, M.S., Philp, J.C., Christofi, N. 1998. Oil desorption from mineral and organic materials using biosurfactant complexes produced by Rhodococcus species. World J. Microbiol. & Biotechnol.