Medium
11
Rhodococcus Surfactant Medium (RS Medium)
Composition per liter:
| K2HPO4 | 2.0 g |
| KH2PO4 | 2.0 g |
| KNO3 | 1.0 g |
| (NH4)2SO4 | 2.0 g |
| NaCl | 1.0 g |
| MgSO4 . 7H2O | 0.2 g |
| CaCl2 . 2H2O | 0.02 g |
| FeCl3 . 7H2O | 0.01 g |
| Trace element solution | 1.0 ml |
pH 6.8-7.0
Preparation of Medium: Yeast extract (0.1 % w/v) is added to the sterilized basal medium. Mixture of n-alkanes (C10-C16 or C12-C17) is used as the sole carbon source at a concentration of 3.0 vol. %. Autoclave liquid media at 121oC for 30 min, agarized - at 105oC for 20 min.
Reference: Ivshina, I.B., Kuyukina, M.S., Philp, J.C., Christofi, N. 1998. Oil desorption from mineral and organic materials using biosurfactant complexes produced by Rhodococcus species. World J. Microbiol. & Biotechnol.