Medium
15
Rhodococcus Medium with ethanol (n-propanol, n-butanol)
Use mineral base of Medium Kievskaya (Malashenko et al., 1973).
Composition per liter:
| KNO3 | 1.0 g |
| KH2PO4 | 1.0 g |
| K2HPO4 | 1.0 g |
| NaCl | 1.0 g |
| MgSO4 | 0.2 g |
| CaCl2 | 0.02 g |
| FeCl3 | 0.001 g |
pH 6.8-7.0
Preparation of Medium: Ethanol (n-propanol, n-butanol) is used as the sole carbon source at a concentration of 1.0 vol. %. Sterilize liquid media at 121oC for 30 min, agarized - at 105oC for 20 min.
Reference: Malashenko, Yu.A., Romanovskaya, V.A., Bogachenko, V.N., Khotian, L.V., Voloshin, N.V. 1973. Characteristics nutrition of microorganisms growing on natural gas. Microbiologiya, 42, 3: 403-408.