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Trypticase soy broth agar

Composition per liter:

Agar 15.0 g
Trypticase Soy Broth 30.0 g

pH 7.3±0.1

Source: Trypticase Soy Broth (BBL 11768, Oxoid CM129 or Merck 5459) is available as a premixed powder from Oxoid.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0 L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 121oC.

Reference: Balows A., Truper H. G., Dworkin M., Harder W., Schleifer K. H. (Ed.). 1992. The Prokaryotes. 2nd Edition. V. III. Springer-Verlag. 4126 p.