Medium
22
Sea water agar
Composition per liter:
| Agar | 20.0 g |
| Beef extract | 10.0 g |
| Peptone | 10.0 g |
| Tap water | 250.0 ml |
| Sea water* | 750.0 ml |
Preparation of Medium: Adissolve beef extract and peptone by heating in tap water, adjust pH to 7.8 and boil for 10 min. Readjust pH to 7.3. Add agar and autoclave at 121oC for 20 min. Directly after autoclaving, add warm (55oC) sterile sea water. Liquid medium without agar should be combined when cooled to room temperature. *Natural sea water is stored in the dark for at least three weeks to "age". If natural sea water is not available use artificial sea water.
*Artificial sea water:
| NaCl | 28.13 g |
| KCl | 0.77 g |
| CaCl2 x 2 H2O | 1.60 g |
| MgCl2 x 6 H2O | 4.80 g |
| NaHCO3 | 0.11 g |
| MgSO4 x 7 H2O | 3.50 g |
| Distilled water | 1000.0 ml |
Reference: Balows A., Downes F. P. and Ito K., (Eds.). 2001. Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C.